مجله دندانپزشکی دانشگاه علوم پزشکی تهران
سال بیست و پنجم شماره 3 (پیاپی 72، پاییز 1391)

There is concern that leached components from dental composites may cause adverse changes in the reproductive health. This study aimed to assess the effects of leached components from a hybrid resin composite on the reproductive system of male mice. In the present animal study, twenty adult Syrian male mice were divided into two groups of 10 mice each. In the test group, components which leached from samples made from Filtek Z250 resin composite into 75% ethanol were daily administered to the mice for 28 days. In the control group, the procedure was repeated in the same way as the test group but without placing composite samples in the solution. Then, the body weight, weights of paired testes, Gonado Somatic Index, sperm viability, sperm motility, epididymal sperm reserve and daily sperm production were recorded. Four male mice in each group were mated with untreated female mice for 10 days. After that, the number of pregnant females and number of infants were recorded. The data were analyzed using repeated measures ANOVA, Chi-square test and t-test. There was a significant reduction in the sperm viability and sperm motility of male mice in the test group compared to the control group (P=0.001). There was no any significant differences in other parameters between two groups (P>0.05). This study showed that the leached components from resin composites cannot cause infertility but they could potentially cause some adverse effects on the reproductive system of male mice.

Periodontitis is one of the most common oral diseases with the various incidence rates in different populations. A number of bacteria are considered as the major etiologic agents of periodontitis. The aim of the present study was to determine the prevalence of periodontopathogen bacteria in patients using both PCR and culture techniques. In this study, one-hundred patients (including 62 females and 38 males with an average age of 49±11.5 years) with adult periodontitis referred to periodontics department of School of Dentistry/Tehran University of Medical Sciences were investigated. The samples were taken and sent immediately to the laboratory for culture and molecular evaluation. The PCR was performed using specific primers and the statistical analysis of data was performed using SPSS statistic software and McNemar test. The results demonstrated that the total detection rate in culture method was 64%. The rate of Aggregatibacter actinomycetemcomitans (Aa) was 28% which was significantly higher than that of Porphyromonas gingivalis (Pg) (6%) and Prevotella intermedia (Pi) (3%). 27% of cases showed mixed bacterial growth. 65% of patients were positive using molecular method. The rate of Aa (30%) was significantly higher than that of Pg (7%) and Pi (5%). The mixed PCR positive rate containing of Aa, Pg and Pi was (23%). In this study, it was found that most of the bacteria isolated using culture and molecular methods were Aa, Pg and Pi, respectively. Although the detection frequencies of both techniques were similar, the specificity, sensitivity and bacterial detection speed of the PCR technique is obviously higher. Therefore, the use of molecular techniques is strongly recommended. However, both techniques seem to be suitable for microbiological diagnostics.

Passive fit of prosthetic frameworks is a major concern in implant dentistry. Impression technique is one of the several variables that may affect the outcome of dental implants. The purpose of this study was to compare the three dimensional accuracy of direct and indirect abutment level implant impressions of ALL-ON-4 treatment plan. A reference acrylic resin model with four Branemark fixtures was made according to All-On-4 treatment plan. Multiunit abutments were screwed into the fixtures and two special trays were made for direct and indirect impression techniques. Ten direct and ten indirect impression techniques with respective impression transfers were made. Impressions were poured with stone and the positional accuracy of the abutment analogues in each dimension of x, y, and z axes and also angular displacement (Δθ) were evaluated using a Coordinate Measuring Machine (CMM). Data were analyzed using T- test. The results showed that direct impression technique was significantly more accurate than indirect technique (P<0.001). The results showed that the accuracy of direct impression technique was significantly more than that of indirect technique in Δθ and Δr coordinate and also Δx, Δy, Δz.

Packable composites with high viscosity might not adapt properly to internal surfaces and cervical areas. The aim of this study was to assess the microleakage of class II posterior composite restorations performed using different methods. Ninety proximal cavities were prepared in extracted sound premolar teeth, divided into three groups and filled as follows: 1- packable composite (3M filtek P60), 2-Hybrid composite (Z250) + P60 composite and 3- Resin-modified glass ionomer liner + P60 composite. Afterwards, the samples were immersed in 0.5% Foushin solution and sectioned. Gingival microleakage was then graded. Obtained data were analyzed using paired t-test and analysis of variance. In regard to distal cavities, significant difference was seen between the groups 1 and 3 (P=0.01) as well as groups 2 and 3 (P=0.03). Comparing microleakage of mesial and distal cavities, there was a significant difference in groups 1 (P=0.003) and 2 (P=0.005). Based on the findings of this study, application of Z250 composite had no effect on reduction of microleakage of class II posterior composite restorations. Vitremer liner decreased microleakage in dento-gingival margins.

Calcium hydroxide has a pronounced antimicrobial activity against most of the bacterial species found in infected root canals and endodontic infections. It is one of the most frequently used intracanal medications in endodontic therapy. The objective of this study was to determine the antimicrobial efficacy of six calcium hydroxide formulations (mixed with saline, lidocaine 2%, chlorhexidine 2%, chlorhexidine 0.2%, Iodine Potassium iodide (IKI) 2%, and glycerin) on Enterococcus faecalis using agar diffusion test. Twelve culture plates were incubated with Enterococcus faecalis. Five cavities were made in each plate with 5 mm diameter and 4 mm depth. Two plates were randomly considered for each calcium hydroxide formulation and filled completely with creamy mixture of tested materials. The plates were incubated at 37ºC for 48 hours. The diameter of inhibition zone around each well was recorded in millimetres and data were submitted to ANOVA and Tukey tests. The results of this study showed that all tested calcium hydroxide pastes had good antimicrobial activity. The antimicrobial activity of calcium hydroxide mixed with lidocaine, chlorhexidine 2% and IKI 2% were significantly greater than that of calcium hydroxide mixed with saline (P<0.001). No significant differences in the antimicrobial were found between the other groups (P>0.01). Considering the results of this study, lidocaine 2%, chlorhexidine 2% and IKI 2% are suggested to be used for preparation of calcium hydroxide paste. Further studies with different methods are needed for confirming these results. Keywords:

Although composite resin restorations have many advantages, they can lead to several clinical problems. The primary reason for these problems is microleakage. The aim of this study was to compare the rebonding effect on microleakage of class V composite restorations using two low viscosity resins. In this in vitro study, 60 class V composite restorations were performed in buccal and lingual surfaces of human extracted premolars with the occlusal margin in enamel and the gingival margin in dentin/cementum. The teeth were randomly divided into 3 groups: (I) restorations with no rebonding, (II) restoration margins rebonded with an enamel adhesive (Margin Bond), (III) restoration margins rebonded with a specific unfilled resin. The specimens were then thermocycled and immersed in 0.5% fuchsin dye solution. The samples were sectioned longitudinally and observed under a stereomicroscope for assessment of microleakage. The data were analyzed by Kruskal-Wallis, Wilcoxon and Mann-Whitney tests. P<0.05 was considered as the level of significance. Statistical analysis showed a significant difference in the microleakage of gingival margins (P<0.0001), but not in enamel margins (P=0.148). Microleakage at the gingival margins of group (III) was significantly less than that of group (II). Group (I) showed the highest amount of microleakage. Rebonding by a low viscosity resin reduces microleakage at the gingival margins of class V composite restorations, but has no significant effect on the occlusal margins.

Electric pulp testing is used for diagnosis of the pulp status. This test is technique sensitive and hence may elicit positive or negative false response in case of inaccurate use. The optimal site for placement of the probe tip has not been determined. The aim of this study was to evaluate the effect of pulp tester probe placement site on the response of maxillary teeth. A total of 378 teeth (126 central incisors, 126 lateral incisors and 126 canines) in 67 voluntary 20-35 year-old patients were selected. Three sites on each tooth (incisal edge, labial and lingual surfaces) were tested with an electrical pulp tester and responses were recorded. Data were analyzed by Repeated Measure ANOVA test. The central teeth showed significantly lower sensation threshold than lateral and canine teeth (P<0.001). The incisal edge of tooth were significantly more sensitive compared to labial and lingual surfaces (P=0.008). This study showed that the optimum site for placement of pulp tester probe was incisal edge.

The purpose of this experimental study was to investigate the shear bond strength of three new adhesive systems to enamel and dentin of permanent human teeth using three new etch and rinse and self-etch adhesive systems. Sixty intact caries-free third molars were selected and randomly divided into 6 groups. Flat buccal and lingual enamel and dentin surfaces were prepared and mounted in the acrylic resin perpendicular to the plan of the horizon. Adhesives used in this study were Tetric N-Bond, AdheSE and AdheSE-One F (Ivoclar/Vivadent, Schaan, Liechtenstein). The adhesives were applied on the surfaces and cured with quartz tungsten halogen curing unit (600 mW/cm2 intensity) for 20 s. After attaching composite to the surfaces and thermocycling (500 cycles, 5-55ºC), shear bond strength was measured using a universal testing machine at a crosshead speed of 0.5 mm/min. The failure modes were examined under a stereomicroscope. The data were statistically analyzed using T-test, one-way ANOVA, Tukey and Fisher's exact tests. In enamel, Tetric N-Bond (28.57±4.58 MPa) and AdheSE (21.97±7.6 MPa) had significantly higher bond strength than AdheSE-One F (7.16±2.09 MPa) (P<0.0001). Tetric N-Bond had higher bond strength than AdheSE but there was not any significant difference between them (P=0.093). In dentin, there were significantly differences among all adhesives (P<0.001). Tetric N-Bond (20.62±3.03 MPa) showed the highest and AdheSE-One F (6.54±1.13 MPa) had the lowest value. The bond strength in enamel was higher than that observed in dentin and the difference was significant in Tetric N-Bond (P=0.044) and AdheSE (P=0.000) groups. There were no significant differences in the mode of failures among groups (P>0.05). Shear bond strength to dentin in Tetric N-Bond (etch and rinse system(was higher than self-etch adhesives (AdheSE and AdheSE-One F). The bond strength to enamel and dentin in two-step self-etch (AdheSE) was higher than one-step self-etch (AdheSE-One F).

The aim of this in vitro study was to assess the shear bond strength of amalgam to dentin using four dentin adhesive systems. One hundred human molars were selected. After enamel removal, a dentin cylinder with 3 mm thickness was prepared. Eighty specimens were resorted with amalgam and four dentin adhesive systems as follows (n=20): group 1, Scotch Bond Multi-Purpose; group 2, One Coat Bond; group 3, PQ1; and group 4, Panavia-F. In group 5, 20 specimens were resorted with amalgam and varnish as control group. The specimens were incubated at 37°C for 24 h. The shear bond strengths were then measured by using push out method. The data were analyzed by one-way ANOVA and post hoc Duncan's tests. Mean values for bond strengths of test groups were as follows: group 1=21.03±8.9, group 2=23.47±9, group 3=13.16±8.8, group 4=20.07±8.9 and group 5=14.15±8.7 MPa±SD. One-way ANOVA showed the statistically significant difference between the bond strengths of five groups (P=0.001). Post hoc Duncan's test showed significant difference between groups 1and 3 (P=0.008), groups 1 and 5 (P=0.019), groups 2 and 5 (P=0.0008), groups 4 and 5 (P=0.042), and groups 3 and 4 (P=0.018). Results of this study showed that the bond strength of amalgam to dentin using One Coat Bond as dentin adhesive system was higher than that observed in other dentin adhesive systems.

Increasing demands for health care's services on one hand and limited resources on the other hand brings about pressure over governments to find out a mechanism for fair and appropriate distribution of resources. Economic analysis is one of the appropriate tools for policy making on this priority. The aim of this study was to assess capital and consumption of oral health units of health care centers in Yazd city and comparing it with revenue of these centers and determining of cost effectiveness. In this descriptive cross sectional study, all health care centers of Yazd city with active dentistry department were evaluated. The data has been extracted from current documents in health care center of county based issued receipts and daily information registers. Expended cost for providing of oral hygiene services in second half of 2008 in 13 medical health centers of Yazd included active dentistry section was 557.887.500 Rials and revenue to cost ratio was about 34%. The most provided service was related to tooth extraction and the average of tooth restoration in each working day was 0.48. With attention to low tariffs of dentistry services in medical health centers and paying subsidy to target groups, expenses of oral hygiene are always more than its revenue.